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Real-time PCR analysis of the expression of IAA11 gene. Seven-day-old seedlings were treated with IAA or CHX with or without PCIB for 2 h. Relative copy number of IAA11 transcripts was calculated by normalizing with number of copies of EF1αA4 transcript. The average of five independent treatments, RNA extraction, and RT-PCR was shown. Bars represent se of the average.
