Figure 5.

Germination frequencies of freshly harvested seeds overexpressing PER1 and wt seeds. A, Immunoblot showing PER1 levels in protein isolated from seeds of wt Arabidopsis and of two pPCV002 35S::Per1 transgenic lines using PER1 antiserum. Ponceau red staining of the membrane was used as a loading control. B, Germination frequencies of seeds from wt and the two pPCV002 35S::Per1 transgenic lines scored 8 d after sowing. Seeds from brown, dried, and just opened siliques were sown at the day of harvest and thereafter every 3rd d until 33 d of after-ripening. Percentages are means of five to eight plants ± sd (in gray for wt and in black for transgenic lines).