Figure 7.

Germination behavior of two AtPER1RNAi lines with reduced levels of AtPER1 mRNA and protein. A, Northern blots of total RNA isolated from 5 DPA and mature seeds were hybridized to an AtPER1 probe and the rRNA loading control probe. The AtPER1 level in protein isolated from mature seeds was detected by western blotting using AtPER1 antiserum. B, Germination on 200 mm mannitol of seeds from the two AtPER1RNAi lines of A compared with wt seeds. Germination was scored at d 2, 4, 6, and 8 of imbibition and is given as the average of three parallel tests ± sd. An average of 265 seeds was tested for each sample in each parallel.