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. 2003 Nov;133(3):1158–1169. doi: 10.1104/pp.103.028183

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Copyright © 2003, The American Society for Plant Biologists

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Figure 2. — Immunoblot analysis of Aox proteins using an anti-Aox antibody. Numbers on the left represent apparent molecular masses in kilodaltons, where the 36-kD band represents GmAox2b, the 34-kD band represents GmAox2a, and the 32-kD band represents GmAox1. A, The profile of Aox proteins present in soybean suspension cells after subculture. Lane numbers indicate the age of the culture in days after subculture. Lane C, contains mitochondria purified from 7-d-old soybean cotyledons. B, Analysis of Aox protein after biolistic transformation. Lane 1 contains d-6 cells before transformation, and lane 2 contains the same cells 48 h after biolistic transformation. Lane 3 contains d-9 cells before transformation, and lane 4 contains the same cells 48 h after biolistic transformation. Lane C contains mitochondria from 7-d-old soybean cotyledons.