Table I.
Sequence motifs common to regions upstream of soybean GmAox2a and 2b and Arabidopsis AtAox1a, -b, and -c
The numbers in parentheses indicate the number of occurrences in the particular upstream region. Motifs identified using motif sampler are given a probability score, where >0.8 is considered significant. Letters other than A, C, G, and T represent degenerate bases, A-T = W, C-G = S, and C-T = Y.
| Motif | Position | Located | Name and Function / Probability Score | Reference |
|---|---|---|---|---|
| Motifs previously characterized in other promoter regions | ||||
| ATGGTA | GmAox1: -1,104; GmAox2a: -675 and -1,110; GmAox2b: -1,227, -1,230, and -660 | AtAox1a (1) and AtAox1b (2), and GmAox1 (2), GmAox2a (2), and GmAox2b (3) | S1F box; cis-acting negative element in non-photosynthetic tissue | Zhou et al. (1992); Lagrange et al. (1997) |
| TGACG | GmAox2b: -800 and -1,025 | AtAox1a (2), AtAox1b (2), and GmAox2b (2) | ASF-1 motif; TGA1a-binding site, high root expression | Benfey et al. (1990); Jupin and Chua (1996) |
| GATAAG | GmAox2a: -130; GmAox2b: -1,000 | AtAox1c (2), GmAox2a (1), and GmAox2b (1) | I Box; cis-acting element involved in light responsiveness | Giuliano et al. (1988); Donald and Cashmore (1990) |
| ATATTTAww | GmAox2a: -890; GmAox2b: -146, -150, -410, and -500 | AtAox1b (1), GmAox2a (1), and GmAox2b(4) | SEF 1 motif; SEF1-binding site, may be involved in young tissue development | Allen et al. (1989); Lessard et al. (1991) |
| Motifs not previously characterized in other promoter regions | ||||
| ATAAAyCTwGCTC | GmAox2b: -70 | AtAox1a (2) and GmAox2b (1) | 0.99 | NA |
| wwwGATAwC | GmAox2b: -290, -310, and -370 | AtAox1a (2) and GmAox2b (3) | 0.87 | NA |
| CGGTswwGA | GmAox2a: -200 and -428 | AtAox1c (2) and GmAox2a (2) | 0.94 | NA |
| wGAAGAwG | GmAox2a: -90 and -320 | AtAox1c (4) and GmAox2a (2) | 0.84 | NA |