Figure 5.

In situ hybridization of antisense and sense QRT3 transcripts to sections of flowers at various stages of microsporogenesis. A to D, Hybridization of QRT3 antisense probe to sections of developing flowers at various stages. E to H, Hybridization of QRT3 sense probes to sections of flowers at the same stages shown in A to E. I and J, Enlarged images of hybridization of antisense QRT3 probe to developing anthers at the tetrad stage. K, Hybridization of QRT3 antisense to ovules of mature flowers. L, Hybridization of antisense eIF4A transcript to section of developing flower at tetrad stage was used as a positive control. The following developmental stages were characterized: A and E, PMC stage buds; B, F, I, and J, tetrad stage buds; C and G, early microspore stage buds; and D and H, late microspore stage buds. Scale bars = 100 μm.