Western blot probed with antibody against QRT3. Approximately 20 μg of extracted protein from various tissues of 4-week old plants was loaded in each lane, blotted to nitrocellulose, and detected with an antibody against QRT3 at a dilution of 1:10,000 (v/v). Lane 1, Wild-type unopened flower buds; lane 2, wild type flowers; lane 3, wild-type expanded leaves; lane 4, wild-type roots; lane 5, qrt3-1 unopened flower buds. The positions of Mr standards are indicated on the left.