Immunohistochemical localization of QRT3 in wild-type flower buds. Ten-micrometer sections of developing anthers were incubated with anti-QRT3 sera at a dilution of 1:1,000 (v/v). A, Tetrad stage; B, early microspore stage; C, late microspore stage; D, mature pollen; E, early microspore stage probed with pre-immune sera; F, early microspore stage probed with secondary antibody. Bars = 100 μm.