Figure 8.

Electropherograms of reaction products from QRT3-mediated hydrolysis of poly-GalUA. Poly-GalUA was incubated with protein extracts from control yeast cells (A) or cells expressing the QRT3 enzyme (B) for 12 h, and then the reaction products were resolved by capillary electrophoresis. The series of peaks between approximately 8 and 9 min in the QRT3 reaction products correspond to the elution times of oligo-GalUA standards containing two to eight residues. Other peaks correspond to contaminants in the dye and by-products of the derivatization reactions.