Table II.

Compared levels of expression of PD73His in the microsomes from yeast strains overexpressing different P450 reductases

Levels of PD73His expression are estimated from the CO-reduced versus reduced difference spectra recorded with the recombinant yeast microsomes. W(R) overexpresses the yeast P450 reductase. WAT11 overexpresses ATR1 from Arabidopsis, WHT1 HTR1 from Jerusalem artichoke, and WVS1 VSR1 from common vetch. All reductases are under the control of the same galactose-inducible promoter (GAL10-CYC1) as PD73His.

Strain	C4H Activity	Level of PD73His Expression		Cyt c Reductase Activity
	pkat mg-1 protein	pmol mg-1 protein	nmol l-1 growth medium	pkat mg-1 protein
W(R)	54.1 ± 1.3	308 ± 6.0	46.8 ± 0.9	742 ± 89
WAT11	157 ± 15.3	369 ± 6.2	37.1 ± 0.6	2,166 ± 28
WHT1	70.1 ± 11.2	313 ± 6.8	26.1 ± 0.5	2,049 ± 135
WVS1	28.6 ± 1.6	179 ± 26	24.3 ± 3.6	1,394 ± 33