Figure 3.

Western blot analysis of PTEN, E2F1 and TGF-βRII production in MSMC, LSMC, t-LSMC and SKLM-S1 with gain- and loss-of-function of miR-21.

Total cellular protein was isolated from cells transfected with 2′-O-MOE anti-miR-21 (A), pre-miR-21 (P) or their respective scrambled oligomer controls (AC and PC) for 48 h and subjected to immunoblot analysis. Note considerable changes in the level of production of PTEN in MSMC and LSMC; E2F1 in t-LSMC and SKLM-S1; and TGF-βRII in MSMC, LSMC and SKLM-S1 (see densitometric analysis in Fig. 4) when compared with controls.