Fig. 1.

Alignment of amino acid sequences of dGGA and three human GGAs. The deduced amino acid sequences of dGGA and hGGAs 1-3 were aligned using the ClustalW program. The regions with purple, yellow and blue underbars indicate VHS, GAT and GAE domains, respectively. Open boxes colored in purple, orange, red and blue indicate amino acid residues involved in the interaction of hGGA1 with ACLL motif, ARF1, ubiquitin and accessory molecules, respectively. The letters in red are putative clathrin-binding motifs and those in blue indicate putative internal dileucine motifs. The ‘*’, ‘:’ and ‘.’ characters indicate positions with fully, strongly and weakly conserved residues, respectively (more detailed information is available at http://align.genome.jp/clustalw/).