Table I.
Relative position of the analyzed restriction sites and methylation sensitivities of the enzymes (Nelson et al., 1993)
| Enzyme | Position Relative to TSSa | Distance from IR Center | Site Cutting | Methylation Sensitivity | Sequence Context |
|---|---|---|---|---|---|
| kb | |||||
| Promoter region approximately 300/+1b | |||||
| TaiI-site a | -79 | ∼1.5 | ACGT | AmCGT | CG |
| TaiI-siteb | -119 | ∼1.6 | ACGT | AmCGT | CG |
| TaiI-sitec | -130 | ∼1.6 | ACGT | AmCGT | CG |
| Sau96-site a | -163 | ∼1.6 | gGGTCCad | GGWmCC, GGWCmC | Nonsymmetrical |
| Sau96I-siteb | -175 | ∼1.6 | gGGACCa | GGWmCC, GGWCmC | Nonsymmetrical |
| AluI | -286 | ∼1.7 | tAGCTg | AGmCT | CWG |
| NcoI | -522 | ∼2.0 | CCATGG | CmCATGG | Nonsymmetrical |
| HpaII | -923 | ∼2.4 | CCGG | CmCGG, mCCGG | CG, CCG |
| MspI | -923 | ∼2.4 | CCGG | mCCGG | CCG |
| CfoI | -1146 | ∼2.6 | GCGC | GmCGC | CG |
| SmaI | -1636 | ∼3.1 | CCCGGG | CCmCGGG | CG |
| Transcribed region +1/+1,035c | |||||
| ClaI | +37 | ∼1.4 | ATCGAT | ATmCGAT | CG |
| BamI-II | +881 | ∼0.6 | gGGATCCt | GGATmCC | Nonsymmetrical |
| SmaI | +884 | ∼0.6 | CCCGGG | CCmCGGG | CG |
| Eco47III | +1,211 | ∼ 0.2 | AGCGCT | AGmCGCT | CG |
a TSS is at nucleotide +1, corresponding to 1,447 bp from the right border.
b The 35S cauliflower promoter securing efficient transcription is approximately 300 bp (Meyer et al., 1994).
c The polyadenylation site from the chalcon synthase gene (accession no. X03710) is at + 1,041, 408 bp from the right border.
d The small letters indicate nucleotides flanking the recognition site that are relevant to the methylation context.