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ECLIPSE monitoring of an oscillatory interaction. (A) The oscillatory interaction between calmodulin and CaMKIIα was validated via ECLIPSE: * indicates the 1st peak. Images were captured at 20 sec. intervals for 30 min. (Scale bars,10 μm). (B) Quantitative analysis confirmed that the oscillatory interaction between calmodulin and CaMKIIα strengthened over time. RFU were normalized against the fluorescence before induction (n = 1). Abbreviations: R, mCherry; IM, ionomycin.
