Table 1. 5-FOA-resistant uracil auxotrophs of H. salinarum NRC-1 with mutations in multiple UMP biosynthetic genes.
Mutations(1) | ||||
Genes analyzed | # of clones sequenced | pyrF | pyrE2 | none |
pyrF | 4 | 2 | 1 | 1 |
pyrE1 | ||||
pyrE2 | ||||
pyrF | 19 | 5 | nd | 14 |
pyrE1 | ||||
pyrF | 16 | 1(2) | 15 | 1 |
pyrE2 | ||||
pyrE1 | 8 | nd | 4 | 4 |
pyrE2 |
no mutation in pyrE1.
mutation in both pyrF and pyrE2.
nd not determined.