Table I.
Phytohormone-related genes: stronger expression in wild-type plants
All transcripts meet the presence criterion in the wild-type expression profile after hybridization of an Affymetrix ATH1 array. The change P values are <0.01 (indicating an increase according to the change algorithm of the MAS 5.0 software). Real-time RT-PCR fold change values were calculated from average threshold cycle (CT) values (see “Materials and Methods”). The table shows average CT values (±se) from three replicates at each experiment for both the gene of interest and eEF1α.
| Affymetrix Identification
|
ATH1 Array: Wild Type vs. dwf1-6
|
Annotation, Description
|
Real-Time RT-PCR: Wild Type vs. dwf1-6
|
|
|---|---|---|---|---|
| Experiment no. 1 | Experiment no. 2 | |||
| Fold change | Fold change, CT values | |||
| Ethylene-related genes | ||||
| 253259_at | 5.3 | At4g34410, putative ethylene-responsive element binding factor | nda | nd |
| 258727_at | 3.5 | At3g11930, putative ethylene-responsive protein | nd | nd |
| 258133_at | 3.0 | At3g24500, putative ethylene-responsive transcriptional coactivator | nd | nd |
| 248799_at | 2.0 | At5g47230, ethylene-responsive element-binding factor 5 (ERF5) | 1.3 | 3.1 |
| WT: CT ERF5 23.19 ± 0.19; CT elF 17.2 ± 0.05 | WT: CT ERF5 21.15 ± 0.36; CT elF 17.33 ± 0.13 | |||
| dwf1-6: CT ERF5 23.38 ± 0.12; CT elF 16.99 ± 0.07 | dwf1-6: CT ERF5 22.68 ± 0.32; CT elF 17.21 ± 0.16 | |||
| 265948_at | 2.0 | At2g19590, putative ACC oxidase | 2.2 | 3.7 |
| WT: CT ACCox 25.19 ± 0.2; CT elF 17.2 ± 0.05 | WT: CT ACCox 25.62 ± 0.12; CT elF 17.33 ± 0.13 | |||
| dwf1-6: CT ACCox 26.1 ± 0.15; CT elF 16.99 ± 0.07 | dwf1-6: CT ACCox 27.4 ± 0.15; CT elF 17.21 ± 0.16 | |||
| 248794_at | 1.7 | At5g47220, ethylene responsive element-binding factor 2 (ERF2) | 2.8 | 7.9 |
| WT: CT ERF2 22.44 ± 0.17; CT elF 17.2 ± 0.05 | WT: CT ERF2 21.49 ± 0.14; CT elF 17.33 ± 0.13 | |||
| dwf1-6: CT ERF2 23.69 ± 0.11; CT elF 16.99 ± 0.07 | dwf1-6: CT ERF2 24.36 ± 0.1; CT elF 17.21 ± 0.16 | |||
| 257922_at | 1.7 | At3g23150, ethylene receptor (ETR2) | nd | nd |
| Auxin-related genes | ||||
| 264929_at | 4.9 | At1g60730, putative auxin-induced protein | nd | nd |
| 262099_s_at | 2.1 | At1g59500, putative auxin-regulated protein GH3 | 6.0 | 2.2 |
| WT: CT GH3 19.96 ± 0.3; CT elF 15.28 ± 0.34 | WT: CT GH3 23.05 ± 0.04; CT elF 17.87 ± 0.05 | |||
| dwf1-6: CT GH3 21.97 ± 0.35; CT elF 14.7 ± 0.07 | dwf1-6: CT GH3 23.3 ± 0.1; CT elF 17.01 ± 0.13 | |||
| 245593_at | 1.9 | At4g14550, Aux/IAA protein family (IAA14) | 2.3 | 1.8 |
| WT: CT IAA14 19.52 ± 0.08; CT elF 15.28 ± 0.34 | WT: CT IAA14 22.8 ± 0.1; CT elF 17.87 ± 0.03 | |||
| dwf1-6: CT IAA14 20.14 ± 0.48; CT elF 14.7 ± 0.07 | dwf1-6: CT IAA14 22.79 ± 0.12; CT elF 17.01 ± 0.13 | |||
| 257766_at | 1.7 | At3g23030, Aux/IAA protein family (IAA2) | 1.7 | 2.6 |
| WT: CT IAA2 17.7 ± 0.03; CT elF 15.28 ± 0.34 | WT: CT IAA2 20.8 ± 0.2; CT elF 17.33 ± 0.37 | |||
| dwf1-6: CT IAA2 17.9 ± 0.1; CT elF 14.7 ± 0.07 | dwf1-6: CT IAA2 22.07 ± 0.07; CT elF 17.21 ± 0.6 | |||
a nd, Not determined.