Table II.
Phytohormone-related genes: stronger expression in the dwf1-6 mutant
All transcripts meet the presence criterion in the dwf1-6 expression profile after hybridization of an Affymetrix ATH1 array. The change P values are <0.01 (indicating an increase according to the change algorithm of the MAS 5.0 software). Real-time RT-PCR fold change values were calculated from average CT values (see “Materials and Methods”). The table shows average CT values (±se) from three replicates at each experiment for both the gene of interest and eEF1α.
| Affymetrix Identification
|
ATH1 Array: dwf1-6 vs. Wild Type
|
Annotation, Description
|
Real-Time RT-PCR: dwf1-6 vs. Wild Type
|
|
|---|---|---|---|---|
| Experiment no. 1 | Experiment no. 2 | |||
| Fold change | Fold change, CT values | |||
| 252677_at | 1.9 | Auxin-related genes At3g44320, hydrolysis of indole-3-acetonitrile to indole-3-acetic acid (NIT3) | 2.9 | 3.0 |
| WT: CT NIT3 26.16 ± 0.09; CT eIF 17.2 ± 0.05 dwf1-6: CT NIT3 24.39 ± 0.26; CT eIF 16.99 ± 0.07 | WT: CT NIT3 23.93 ± 0.19; CT eIF 17.33 ± 0.13 dwf1-6: CT NIT3 22.21 ± 0.13; CT eIF 17.21 ± 0.16 | |||
| 252184_at | 2.5 | BR-related genes At3g50660, steroid 22-hydroxylase (DWF4) | 2.2 | 1.9 |
| WT: CT DWF4 23.87 ± 0.19; CT eIF 17.2 ± 0.05 dwf1-6: CT DWF4 22.54 ± 0.06; CT eIF 16.99 ± 0.07 | WT: CT DWF4 23.8 ± 0.04; CT eIF 17.33 ± 0.13 dwf1-6: CT DWF4 22.77 ± 0.08; CT eIF 17.21 ± 0.16 | |||
| 250752_at | 1.3 | At5g05690, steroid 23-hydroxylase (CPD) | nda | 1.9 |
| WT: CT CPD 24.38 ± 0.1; CT eIF 17.8 ± 0.07 dwf1-6: CT CDP 23.76 ± 1.0; CT eIF 18.1 ± 0.32 | ||||
a nd, Not determined.