Skip to main content
. 2010 Feb 4;5(2):e9063. doi: 10.1371/journal.pone.0009063

Table 4. Sequences for the binding elements and their mutated counterparts used in the EMSA analysis.

SREBP-1 FP 5′ GATCCTGATCACCCCACTGAGGAG 3′
SREBP-1 RP 5′ CTCCTCAGTGGGGTGATCAGGATC 3′
SREBP-1 mutated FP 5′ GATCCTGATCTCCCGACTGAGGAG 3′
SREBP-1 mutated RP 5′ CTCCTCAGTCGGGAGATCAGGATC 3′
FOXJ2 FP 5′ CACATAAACAAATACATCA3′
FOXJ2 RP 5′ TGATGTATTTGTTTATGTG 3′
FOXJ2 mutated FP 5′ CACGTAAGCAAATACATCA 3′
FOXJ2 mutated RP 5′ TGATGTATTTGCTTACGTG 3′

FP: Forward Primer.

RP: Reverse Primer.

Poistions of alterations in the mutated oligonucleotides within the binding motifs are shown by bold and underlined bases.