Table 4. Sequences for the binding elements and their mutated counterparts used in the EMSA analysis.
SREBP-1 FP | 5′ GATCCTGATCACCCCACTGAGGAG 3′ |
SREBP-1 RP | 5′ CTCCTCAGTGGGGTGATCAGGATC 3′ |
SREBP-1 mutated FP | 5′ GATCCTGATCTCCCGACTGAGGAG 3′ |
SREBP-1 mutated RP | 5′ CTCCTCAGTCGGGAGATCAGGATC 3′ |
FOXJ2 FP | 5′ CACATAAACAAATACATCA3′ |
FOXJ2 RP | 5′ TGATGTATTTGTTTATGTG 3′ |
FOXJ2 mutated FP | 5′ CACGTAAGCAAATACATCA 3′ |
FOXJ2 mutated RP | 5′ TGATGTATTTGCTTACGTG 3′ |
FP: Forward Primer.
RP: Reverse Primer.
Poistions of alterations in the mutated oligonucleotides within the binding motifs are shown by bold and underlined bases.