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. 2010 Feb 4;5(2):e9064. doi: 10.1371/journal.pone.0009064

Figure 5. Akt activation by SM is required for induction of meiotic entry of Kit positive spermatogonia.

Figure 5

A) Representative western blot of Erk1/2 and Akt phosphorylation state in Kit positive spermatogonia from 7dpp testes, cultured for 24 h in RCCS. Spermatogonia stimulated with KL for 15 min were used as positive control. Densitometric quantitation is shown in the right panel. B) Western blot analysis showing a decrease of phospho-Akt and Scp3 in spermatogonia cultured for 24 h under SM in the presence of 10 µM LY294002. Similar results were obtained in three separate experiments. Densitometric quantitation is shown in the right panels. C) Histograms representing the percentage of nuclei with a meiotic Scp3 organization (preleptotene and early leptotene morphology) after 24 h of culture at unit gravity and in SM, in the presence or absence of the Akt inhibitor LY294002. The values were obtained by counting 500 nuclei in each sample. Data are means ± SD of three independent experiments. P≤0.005.