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. 2010 Feb 4;5(2):e9046. doi: 10.1371/journal.pone.0009046

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Larzábal et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Hemolysis of sheep RBC observed with EPEC bacteria preincubated with coiled-coil peptides. Results are presented as percentage relative to hemolysis (± standard deviation) observed upon incubation of RBC with EPEC alone. K12 means the hemolysis caused by the E. coli non-pathogenic strain. (B) Doses-dependent effect of CoilA and CoilB peptides on the hemolysis of RBC. The peptides were applied to the RBC-E2348/69 system to various concentrations and a dose-dependent response was observed. pH represent a mock control to rule out the biological influence of alkaline pH used to dissolve peptides. Each assay was done by triplicate.