Figure 4. CoilA and CoilB peptides block EspA polymerization and Tir translocation.

Cell monolayers were infected with bacteria pretreated with peptides, washed, and lysed for Western blot detection of EspA and Tir proteins with specific rabbit antisera. pH represents a mock control to rule out the biological influence of alkaline pH used to dissolve peptides; PBS at alkaline pH was used in a volume identical to those in which peptides were added to HEp-2 cells.