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Membrane association of LGDT in S. flavescens cultured cells
Preparation of fractions and LGDT assay are described in “Materials and Methods.” Data are the means of duplicate determinations ± se.
| Enzyme Solution | Total Activity | Total Protein | Specific Activity |
|---|---|---|---|
| pkat g cells-1 | mg g cells-1 | pkat mg protein-1 | |
| Crude cell-free extracts | 17.08 ± 0.30 | 7.10 ± 0.49 | 2.41 ± 0.04 |
| 156,000g supernatant | 0.85 ± 0.45 | 5.79 ± 0.48 | 0.15 ± 0.09 |
| Microsomal fraction | 14.23 ± 0.63 | 0.52 ± 0.02 | 27.37 ± 1.21 |
