Fig. 3.

Lanes 1–5 of whole-cell extracts (WCEs) of clinical isolates of M. tuberculosis. a, b Blotting with BCG-vaccinated healthy individual’s sera. c–d Reactivity with individual tuberculosis patient’s sera. e Reactivity pattern of contact with tuberculosis. f 12% SDS–PAGE gel pattern of WCEs of clinical isolates and H37Rv. g Hybridization of polyclonal antibody against ESAT-6 and CFP-10 with whole-cell extracts. h Hybridization of monoclonal antibody (CS-90) against Ag85 complex with WCEs of clinical isolates and H37Rv. Rv: M. tuberculosis laboratory reference strain H37Rv; arrow shows the reactivity pattern of Ag85 complex