Table III.
Metabolite levels in illuminated leaves of 6-week-old plants of the tomato genotypes
Leaves were harvested 6 h into the photoperiod and irrespective of the method of analysis, all metabolite analyses were performed from the same tissue samples. Values presented are the mean ± se of measurements from six plants per genotype. Amino acid values in parentheses were determined in samples taken in the dark period. Organic acid contents, normalized as described in “Materials and Methods,” are also presented as the mean ± se of measurements from six plants per genotype. Asterisks and bold type indicates those that were determined by the Student's t test to be significantly different between genotypes.
| Metabolite | Lp | Aco-1 |
|---|---|---|
| Organic acids | ||
| Succinate | 1.0 ± 0.3 | 0.4 ± 0.1* |
| Isocitrate | 1.0 ± 0.3 | 1.4 ± 0.3*a |
| Citrate | 1.0 ± 0.1 | 1.5 ± 0.1* |
| α-kt-glutarate | 1.0 ± 0.4 | 0.3 ± 0.4* |
| Malate | 1.0 ± 0.5 | 0.9 ± 0.2 |
| Fumarate | 1.0 ± 0.1 | 0.5 ± 0.1* |
| nmol g fresh wt-1 | ||
| Amino acids | ||
| Asp | 308.2 ± 29.0 (222.9 ± 3.3) | 149.9 ± 12.2* (182.9 ± 33.4) |
| Glu | 732.8 ± 207.5 | 729.5 ± 75.4 (476.3 ± 112.7)* |
| (737.9 ± 91.7) | ||
| Asn | 141.5 ± 16.5 (163.8 ± 24.3) | 228.2 ± 40.6* (105.9 ± 19.7) |
| Ser | 138.6 ± 22.8 (144.9 ± 19.3) | 166.1 ± 40.1 (105.8 ± 19.1) |
| Gln | 264.3 ± 59.9 (259.3 ± 50.8) | 206.1 ± 51.4* (164.6 ± 47.0)* |
| Gly | 98.4 ± 26.82 (n.d.b) | 184.3 ± 30.9* (n.d.) |
| Arg | 124.9 ± 29.1 (148.7 ± 4.5) | 100.1 ± 9.9 (72.2 ± 11.3)* |
| Phe | 49.6 ± 18.7 (46.6 ± 9.4) | 28.5 ± 1.9* (22.7 ± 6.3)* |
| Leu | 32.5 ± 11.1 (11.7 ± 1.9) | 19.3 ± 0.9* (8.5 ± 1.3) |
| Nucleotides | ||
| ATP | 11.7 ± 2.7 | 19.7 ± 2.0 |
| ADP | 21.3 ± 3.1 | 44.0 ± 11.5* |
| ATP/ADP | 0.6 ± 0.0 | 0.4 ± 0.0* |
| Σ Adenylate | 32.3 ± 7.0 | 59.7 ± 18.3* |
| UDP-Glc | 4.7 ± 0.8 | 15.6 ± 5.6* |
| UTP | 15.9 ± 2.5 | 17.7 ± 3.9 |
| UDP | 4.7 ± 0.9 | 0.37 ± 0.0* |
| UTP/UDP | 3.7 ± 0.5 | 30.0 ± 3.6* |
| ATP/UTP | 0.9 ± 0.2 | 1.0 ± 0.2 |
| Sugar phosphate | ||
| 3 phosphoglycerate (3PGA) | 262.0 ± 23.2 | 316.0 ± 16.8* |
| Glucose 6-phosphate | 106.4 ± 4.9 | 137.2 ± 5.6* |
| Glucose-1-phosphate | 19.6 ± 3.3 | 25.1 ± 2.2 |
| Fructose 6-phosphate | 19.8 ± 1.6 | 24.7 ± 4.3 |
| Σ hexose-P | 145.8 ± 8.5 | 187.0 ± 9.9* |
| Pi | 5.3 ± 1.8 | 5.8 ± 0.6 |
| Σ Phosphates (inorganic phosphate) Pi | 75.5 ± 1.3 | 86.9 ± 2.1* |
| 3PGA/Pi | 48.6 ± 5.7 | 54.6 ± 3.95* |
a Isocitrate was measured following the protocol described by Beutler (1989).
b n.d., Not detectable.