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Activity of PRT1 is inhibited by O-methyl esters of destabilizing amino acids. Enzyme activity measurements similar to those of Figure 1 were carried out after growth of yeast cells in the presence of Phe-O-methyl ester, Trp-O-methyl ester, Leu-O-methyl ester, and Arg-O-methyl ester. Only those methyl esters which correspond to an amino-terminus destabilized by PRT1 expression can inhibit the degradation process.
