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. 2007 Oct 29;7(1):63–68. doi: 10.1093/ecam/nem146

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© 2007 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Phosphorylation of CREB in PC12 cells after treatment with various agents. PC12 cells were cultured for 30 min in medium supplemented with various concentrations of AMP N₁-oxide (A) or in medium supplemented or not with 20 μM AMP N₁-oxide in the presence or absence of various concentrations of KT5720 or ZM 241385 for 30 min (B). The cell lysates were subjected to PAGE (5 μg protein/lane) and blotted onto membranes for Western blotting with anti-phosphorylated-CREB and anti-CREB antibodies.