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Comets analysis by fluorescence microscopy with visual inspection of tail length of nuclei. The cell nuclei were classified into five categories: (0) undamaged (nuclei without comet tail); (1) low damaged (nuclei with comet tails up to two fold longer than nucleus diameter); (2) damaged (nuclei with comet tail two to three fold longer than nucleus diameter), (3) highly damaged (nuclei with comet tails three fold longer than nucleus diameter), and (4) severely damaged, respectively.
