Figure 3.

Adhesion of neutrophils to immobilized fibronectin leads to delayed membrane translocation of Rac2, but not p47 and p67. Neutrophils plated on immobilized fibronectin or in nonadherent conditions were treated with 1 × 10^–7 M fMLF. After the times indicated, the cells were fractionated as in Methods, and the presence of Rac2, p47, p67, and RhoGDI on the cell membrane were analyzed. The last lane of the GDI panel represents 10 μg of cytosol as a RhoGDI antibody control. Results shown are representative of three independent experiments using cells from different donors.