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. 2003 Dec 1;112(11):1603–1618. doi: 10.1172/JCI20401

Figure 5.

Figure 5

Chromosome nonsegregation and aneuploidy results from failure of PTTG-EGFP fusion protein degradation after transfection of a degradation-deficient PTTG mutant. (a) Single live H1299 cells with persistent PTTG-EGFP expression were continuously observed and show absence of chromosome segregation with completed cytokinesis. Phase contrast (bright field) and PTTG-EGFP images (green) are shown; D2, second day of observation. Arrow, cell entering mitosis. Scale bar, 10 μm. (b) Aneuploidy correlation with transfected PTTG expression levels or with failure to degrade PTTG in a degradation-deficient mutant. C, control. Panel a modified with permission from Endocrinology (129).