Table 4.
β-Carotene bleaching inhibitory activity of different fractions of Coccinia grandis
| Fractions | Time of incubation (min) |
Absorbance at 470 nm | IC50 (mg/ml) |
||||
| 50 µg/ml |
100 µg/ml | 200 µg/ml | 400 µg/ml | 800 µg/ml | |||
| Pet-ether | 0 | 0.282 | 0.397 | 0.562 | 0.609 | 0.695 | 0.068 a ±0.04 |
| 90 | 0.089 | 0.166 | 0.295 | 0.328 | 0.391 | ||
| Chloroform | 0 | 0.075 | 0.089 | 0.099 | 0.119 | 0.145 | 0.117 b ±0.01 |
| 90 | 0.047 | 0.054 | 0.062 | 0.080 | 0.104 | ||
| Ethyl acetate | 0 | 0.415 | 0.632 | 0.713 | 1.208 | 1.283 | 0.099c ±0.01 |
| 90 | 0.288 | 0.407 | 0.447 | 0.924 | 0.983 | ||
| Residual | 0 | 0.515 | 0.575 | 0.642 | 0.715 | 0.858 | 0.145 d ±0.04 |
| 90 | 0.455 | 0.502 | 0.557 | 0.622 | 0.763 | ||
| Ascorbic acid | 0 | 0.091 | 0.137 | 0.189 | 0.285 | 0.725 | 0.140 e ±0.06 |
| 90 | 0.059 | 0.082 | 0.138 | 0.230 | 0.664 | ||
Values are expressed as mean± SEM of three parallel measurements. Values within a column followed by different letters are significantly different (P<0.05).