Skip to main content
. 2009 Dec 11;11(1):45–51. doi: 10.1038/embor.2009.256

Figure 3.

Figure 3

Nix recruits EGFP-GABARAP-L1 to stressed mitochondria in reconstituted Nix−/− MEFs. Nix−/− MEFs cotransfected with EGFP-GABARAP-L1 and wt-Nix (A), or Nix-W35A (B), were incubated with and without mitochondrial poison CCCP for 3 h. Cells were then fixed and analysed for colocalization between EGFP-GABARAP-L1 and Nix (stained with Flag antibodies). The final panel in each row shows colocalization between Flag-Nix-Cy3 and GABARAP-L1 using the ‘colocalization' highlighter plug-in for ImageJ software (NIH, Bethesda, MA, USA). (C) Quantification was achieved by counting the number of complete colocalizations, EGFP/Cy3 per cell for approximately 30 cells per condition. Results are expressed as a percentage of colocalization (EGFP:Cy3) in control and CCCP-treated cells. Error bars in (C) show the standard deviation (s.d.) of three independent experiments. Statistical analysis was performed using the Mann–Whitney t-test. CCCP, carbonyl cyanide m-chlorophenyl hidrazone; DMSO, dimethyl sulfoxide; EGFP, enhanced green fluorescent protein; MEF, mouse embryonic fibroblast; wt, wild type.