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. 2009 Dec 8;102(2):403–413. doi: 10.1038/sj.bjc.6605481

Figure 1.

Figure 1

Poly-unsaturated fatty acid (PUFA) components, but not heterogeneous dietary oils, are stimulators of prostate cancer (CaP) invasion. 2 × 105 PC-3-GFP cells were seeded in modified Boyden chambers above either (A) 1 μg ml−1 dietary lipids or increasing levels of (B) linoleic acid (LA) (C) α-linolenic acid (ALA) (D) oleic acid encaged in methyl-β-cyclodextrin. Levels of invasion are proportional to fluorescence detected by a bottom reading BMG FLUOstar OPTIMA plate reader at 488/520 nm (excitation/emission filter). In each assay (n=3) a serum free RPMI 1640 (TCP)–ve control, human BMS+ve control and a 3 μg ml−1 arachidonic acid (AA) control were included.