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. 2010 Jan 5;102(2):325–331. doi: 10.1038/sj.bjc.6605500

Figure 3.

Figure 3

Interaction between MRGBP and BRD8. (A) COS7 cells were transfected with pcDNA-Myc/His-MRGBP, pCAGGS-HA-BRD8, or the combination of the two. Extracts from these cells were immunoprecipitated with anti-HA (upper two panels) or anti-Myc antibody (lower two panels). (B) Nuclear extract from SW480 cells were immunoprecipitated with anti-MRGBP (upper two panels) or anti-BRD8 antibody (lower two panels). Western blot analysis was performed using the indicated antibodies. (C) Interaction of wild type or the deletion mutants of MRGBP with BRD8. HEK293 cells were transfected with HA-tagged BRD8 plasmid and wild type or several deletion mutants of Myc-tagged MRGBP plasmids. Extracts from these cells were immunoprecipitated with anti-Myc antibody and then immunoblotted with anti-HA antibody (upper panel). Expressions of HA-tagged BRD8 and β-actin were shown in the middle panel and the lower panel, respectively.