Skip to main content
. 2010 Jan 5;102(2):325–331. doi: 10.1038/sj.bjc.6605500

Figure 4.

Figure 4

MRGBP increases expression of BRD8 protein in a post-transcriptional manner. (A) HEK293 cells were transfected with pcDNA-Myc/His-MRGBP and/or pCAGGS-HA-BRD8. After transfection, the cells were harvested at the indicated time points, and western blot analysis was performed (upper two panels). Expression of β-actin served as a control. Simultaneous northern blot analysis using BRD8 or β-actin cDNA as a probe was performed (lower two panels). Expression of β-actin served as a control. (B) Effect of knockdown of MRGBP on BRD8 expression. HCT116 cells were transfected with MRGBP-specific or control siRNA for 72 h. Nuclear extracts were isolated, and western blot analysis was performed using the indicated antibodies. Expression of Histone H3 served as a control.