Table 2.

T_m values of 7E15 variants

Protein	Tm (°C)
	EGTA	Ca2+	Tb3+
CD2	61 ± 1	61 ± 1	-
EEDDD	41 ± 1	51 ± 1	-
EEDDE	44 ± 2	56 ± 1	63 ± 2
EEDDN	52 ± 2	58 ± 2	54 ± 1
EEDDQ	56 ± 1	61 ± 1	60 ± 1
EENDN	61 ± 2	59 ± 2	59 ± 2
NENDN	60 ± 3	58 ± 2	58 ± 4

Using far-UV CD all T_m values were obtained in 10 μM protein solution with 1 mM EGTA, 10 mM Ca²⁺, and 0.1 mM Tb³⁺, respectively. The buffer system is 10 mM Tris, 10 mM KCl, and pH 7.4.