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. 2010 Feb 5;6(2):e1000835. doi: 10.1371/journal.pgen.1000835

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Du et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Purified full CENPC and different sized CentC DSDNA binding substrates (indicated at top). The binding efficiency as % binding is shown (bound DNA/total). (B) Competition experiments. Lanes were loaded with the mixture of full CENPC protein, 44 bp CentC DSDNA, and either unlabeled 44 bp CentC or 44 bp Ndc80 DSDNA. (C) Increasing quantities of CENPC cause a supershift on a 44 bp substrate. (D) Increasing quantities of CENPC do not cause a supershift on the 24 bp substrate. The triangles represent the amount of protein or DNA added, and arrows indicate the shifted bands.