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. 2010 Feb 5;6(2):e1000835. doi: 10.1371/journal.pgen.1000835

Figure 4. The purified Exon 9–12 domain requires RNA to bind DNA in vitro.

Figure 4

(A) The DNA binding domain of CENPC alone does not bind to DNA, however a shifted band becomes evident as increasing amounts of SSRNA are added. The same is true for Exon 9–10 (B) and exon 12 alone (C). (D) Only single-stranded RNA or DNA stabilizes Exon 9–12 for DSDNA binding. Gel shift is observed in the presence of 24 nt SSDNA, 24 nt SSRNA, and 48 nt poly-GT (SSDNA). However, no shift is observed for 44 bp DSDNA, 167 nt RNA (with double stranded character) or 10 nt RNA. (E) Sequence of CENPC exon 12.