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. 2010 Feb 5;6(2):e1000833. doi: 10.1371/journal.pgen.1000833

Figure 4. Knockdown of SLC29A3 expression by siRNA enhanced proliferation of HeLa cells.

Figure 4

(A) Effect of SLC29A3 siRNA on levels of SLC29A3 mRNA assessed by real-time quantitative PCR (qRT–PCR). HeLa cells were treated with control or SLC29A3- 120032 or SLC29A3-26642 siRNA for 72 hours. Cells were harvested and total RNA was extracted. SLC29A3 and β-actin or SLC29A3 and β-2-microglobulin mRNA (was examined by qRT-PCR. Values are mean ± SEM from 3 controls and 3 samples. * P<0.001 between HeLa cells treated with SLC29A3-siRNA versus luciferase siRNA control sequence. (B) SLC29A3 knockdown elevates proliferation in HeLa cells. HeLa cells were transfected with siRNA designed to target SLC29A3 (siRNA-SLC29A3) or control siRNA. Cell proliferation assays were performed 72 hours after siRNA transfection. Results areexpressed in fluorescence at 550 nm using 580 nm as a reference wavelength(fluorescence is directly proportional to the number of living cells). This figure represents 3 experiments.* P<0.05.