Figure 5. Expression of SLC29A3 mRNA in the mouse embryo.

Sagittal sections from e14.5 embryos were processed for in situ hybridisation followed by imaging of representative organs (hybridization signal corresponds to blue and red/pink is a general counterstain). While there is a level ubiquitous expression throughout the embryo, several areas within the central and peripheral nervous system showed increased expression levels particularly in the dorsal spinal cord (meninges and skin to the top) (A), dorsal anterior forebrain (B), dorsal posterior midbrain (C), dorsal root ganglia (D), trigeminal ganglion (E), dorsal root ganglia (E), eye and anterior lens surface (F), ear (G), choroid plexus (H), and olfactory epithelium (I). Within trunk organs localised expression is seen in the developing lung bronchioles (J), glomeruli of the kidney cortex (L), early pancreatic primordial (M), gonads (N), thymus (O), internal mucosa of the gut (P) and stomach (Q), and lower level is the liver (K). Increased SLC29A3 expression is also seen in the outer epidermal layer of the developing posterior ventral trunk skin (R). At e14.5 trunk hair follicles are not well-developed compared, however, snout vibrissae (S,T) show elevated expression levels (a glancing more longitudinal section of an individual vibrassa can be seen in T). Scale bar in T is 100 um and applies to all images.