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. 2010 Feb 5;5(2):e9073. doi: 10.1371/journal.pone.0009073

Table 1. Primers sequences used to amplify and sequence each locus.

Locus Gene product PCR primer sequences5′→3′ Sequencing primer sequences5′→3′
accC acetyl-CoA carboxylase- biotin carbolylase subunit accC-F accC-R TGTGCGTATCATTC GCATCACCCATTTTACCACG TTGTGACTGTTGCAGT GCAATGAACAATCACG
gki glucokinase gki-F gki-R GTCCTCTATCAAGCATCG TATCACCTGCCTGTGCAGCA GTCCTCTATCAAGCATCG ACTGCTCACATCATCACC
lepA GTP-binding protein lepA-F lepA-R CTCTATTATTGCCC TACATCACCCGTTG ATGGGAAGTCAACGCT GACCATACCATCCAT
recP transketolase recP-F recP-R AAGTCTAAGTCGGGTC TGACCAAGCATCGTAAGCAG CTGTTGGTTTGGCTCA TGACCAAGCATCGTAAGCAG
sodA superoxide dismutase sodA-F sodA-R GGCTATTCTTTTACCAG GGCATAAAGACGAGCAACAG GCTTATGACGCACTTGAACC GGCATAAAGACGAGCAACAG
tyrS tyrosyl-tRNA synthetase tyrS-F tyrS-R CCAACAGCAGATAG CCACGACGAATAAC GAGGCATTTACAATTGGC AGCCCCGTTTTGAAGA
gtfB glucosyltransferase gtfB-F gtfB-R GGACAAGAAAGTGCGT AGTTTGATTGGTCGGG CTGCTGTGATGACTT TACCAACTTTCGGCTGTC
spaP surface protein antigen spaP-F spaP-R AGTGCGAGTAAGGAAG TTCACCGCTGCCAAAT TGCTAAGTCTGCTGGTGTCA GCT(CT)GATAGTCTGCTTCG