Skip to main content
. 2010 Feb 5;5(2):e9095. doi: 10.1371/journal.pone.0009095

Figure 4. Proteoglycan core proteins expressed in transplant vasculopathy, glomerulosclerosis and interstitial fibrosis in allografts contain functional glycosaminoglycan side chains.

Figure 4

Neointimal cells in TV (A), glomerular BMs in non-sclerotic areas (B) and tubular BMs in IF (C) express heparan sulfate domains with N-unsubstituted glucosamine residues as recognized by antibody JM-403. Following heparitinase treatment presence of heparan sulfate stub regions was identified in medial and neointimal cells in TV (D), in glomerular BMs (E) and in tubular BMs (F) using antibody F69-3G10. Dotted line in panel A and D represents the internal elastic lamina. Abbreviations: M: media; NI: neointima. L-selectin-IgM chimeric protein binding in the tubulointerstitium in no pre-treated sections (G), sections pre-treated with heparitinase I [hep I] (H), sections pre-treated with chondroitinase ABC [chonABC] (I) and sections pre-treated with both heparitinase I and chondroitinase ABC (J). Insets show high power magnifications of the framed areas. Arrows: tubular BMs, asterisks: interstitium.