Fig. 5.
Denaturant dependence of the folding/unfolding rate constants of Im7H3M3, together with the initial and final fluorescence signals for folding. Top: The observed rate constants (circles) for the folding/unfolding of Im7H3M3 plotted as a function of urea concentration. The data were obtained at pH 7.0, 10 °C and 0.4 M Na2SO4. The continuous line shows the best fit of the data to a model describing a three-state transition. The broken line shows the data for wild-type Im7. Bottom: The initial (triangles) and final (circles) fluorescence signals for the folding of Im7H3M3. The continuous lines show the initial and final fluorescence signals fitted simultaneously with the rate constants. The broken line represents the best fit of the data obtained for wild-type Im7.