Figure 2.

Jarid2 regulates PRC2-Ezh2 enzymatic activity. (A) HKMT assay with PRC2-Ezh2 alone or in the presence of increasing amounts of Jarid2 or Rbp2. Recombinant oligonucleosomes (top) or octamers (bottom) were used as substrate. (B, top) Schematic representing the known Jarid2 domains. Also shown are the regions of Jarid2 protein that were expressed in bacteria. (Bottom) Band shift assay in the presence of increasing amounts of each of the Jarid2 fragments using the radiolabeled DNA probes selected by SELEX. The asterisks indicate nonspecific signals present in the free probes. The arrow indicates the specific band shift. (C) Coimmunoprecipitations of purified Ezh2 protein and Jarid2 fragments, as indicated. Immunoprecipitation was performed using the Ezh2 antibody. (D) HKMT assay with PRC2-Ezh2 alone or in the presence of increasing amounts of fragment 2 or fragment 4 of Jarid2 using oligonucleosomes (left) or octamers (right) as substrate.