Fig. 4.
Dose–activity curves with low (a) and high (b) Hill coefficients. The enzymatic activity of the phosphatase MPTPB was related to the activity in the absence of inhibitor (100%). The background was measured from the signal in the absence of enzyme. The data (+) were obtained from a dilution series (by a factor of 2). Blue line Data fit to competitive 1:1 inhibition (nH = 1), green line four-parameter logistic fit. Dashed red line Data fit to reaction Eq. 9 with parameters (highlighted in bold letters) listed in Table 1. a Compound 6555, nH = 0.56, optimal fit with the conditions 2 and 4. b Compound 40763, nH = 1.82, optimal fit with the conditions 2 and 3