Table 3.
Congo Red binding to various amyloid fibril in vitro
| Type of fibrils | na | Kd (μM) | Measurement | Reference(s) |
|---|---|---|---|---|
| Aβ(1–40) fibrils (pH 7.4) | 0.60 | 1.1 (0 M NaCl) | Abs (centrifugation) | [148] |
| 2.6 | 1.5 (0.1 M NaCl) | Abs (centrifugation) | [148] | |
| 0.43 (0 M NaCl) | – | Abs (centrifugation) | [149] | |
| 0.89 (0.14 M NaCl) | – | Abs (spectrophotometric) | [39] | |
| α-Poly-l-lysine (pH 7.4) | 36 | – | Abs (filtration) | [140] |
| β-Poly-l-lysine (pH 11) | 42 | 0.51 | Abs (filtration) | [140] |
| Insulin fibrils (pH 7.4) | 1.8 | 0.13 | Abs (spectrophotometric) | [38, 140] |
| 2.2 | 0.10 | Abs (filtration) | [38, 140] | |
| Poly-l-serine (pH 7.4) | 0.12 | 0.42 | Abs (filtration) | [140] |
| Polyglycine (pH 7.4) | 0.33 | 0.82 | Abs (filtration) | [140] |
Abs is absorbance and centrifugation, spectrophotometric and filtration refer to different methods applied to measure the concentration of bound dye. n is number of binding sites, while Kd is dissociation constant. – no data
aMoles of CR per mole of protein in fibril form