Fig. 5.

Axial CLSM projections are shown of the injured cartilage edge and PCD_SZ as a function of increasing saline osmolarity (100–600 mOsm). The panels show axial CLSM projections of the articular surface. The graph shows the corresponding pooled data for PCD_SZ as a function of increasing saline osmolarity. The band of superficial zone chondrocyte death at the cut cartilage edge (PI-stained red nuclei) decreases for explants exposed to increasing osmolarity of the saline solution (N = 3, n = 36, white bar = 100 μm). CLSM = confocal laser scanning microscopy; PCD = percentage cell death; PI = propidium iodide.