Fig. 1.
Dose-dependent growth stimulation by meconium in amniotic fluid (AF). About 1×105 cfu/mL of the preconditioned S. aureus ATCC 6538 was inoculated into the iron-sufficient BHI (IS-BHI) and AF containing 1, 5 and 10 mg/mL of meconium, respectively, and cultured with vigorous shaking (200 rpm) at 37℃ for 12 hr. At the indicated intervals, bacterial growth was measured by optical density at 600 nm of wavelength. Experiments were carried out at least three times with similar results, and a representative one is shown.