Fig. 6.
Growth according to the addition of various iron sources into the iron-deficient (ID) BHI. Two bacterial strains were used: S. aureus ATCC 6538 standard strain and streptonigrin-resistant (SR) strain. About 1×105 cfu/mL of the bacteria was inoculated into the ID-BHI (C) containing 0.5 mg/ mL of apotransferrin (A), 0.5 mg/mL of holotransferrin (B), 5 µM of FeCl3 (D) and 0.5 mg/mL of hemoglobin (E). Bacterial growth was measured by optical density (OD) at 600 nm of wavelength at the indicated intervals. Experiments were carried out at least three times with similar results, and a representative result was shown.