Figure 2.

TLR2 ligand co-stimulates T cell proliferation in the total T, CD4, and CD8 T cells. Total T cells were isolated as described in Materials and Methods and incubated with anti-CD3s in the presence or absence of TLR2 ligand Pam₃CSK₄ (PAM) for 64 h. (A) The T cell proliferation was determined via [³H]thymidine incorporation. Values are means and standard deviations of data from three independent experiments. ^**p<0.001, anti-CD3 vs. anti-CD3+PAM. (B) Cultured cells were treated with BrdU (2 µg) for the last 1 h and stained with PE-anti-CD4 or -CD8 mAb. The cells were then stained for incorporated BrdU and analyzed by flow cytometry. The histogram for BrdU incorporation was gated on CD4⁺ or CD8⁺ cells.