Figure 5.

IFN-γ plus 1,25-D3 induces antimycobacterial activity against MTB. (A) Densitometric analysis for cathelicidin (LL-37) mRNA expression in human MDMs stimulated by PPD after an 18-h pretreatment with IFN-γ, 1,25-D3, or both. Cathelicidin levels are expressed relative to β-actin levels. (B, C) Antimicrobial activity by human MDMs pretreated with IFN-γ, 1,25 D3, or both. Cells were pre-incubated for 18 h and infected with MTB H37Rv (MOI=1). At day 7, the number of viable bacteria was assessed by CFU assay. Results are representative of seven experiments. (C) At the time points shown, the cells were lysed, and [³H]-uracil incorporation into the released bacteria was measured. Results are representative of six experiments. ^*p<0.05, ^**p<0.01, ^***p<0.001 vs. MTB H37Rv-treated control.